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    Reading NGS Reports: VAF, Actionable Mutations, VUS & More

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    FAQ #1 –

    How should low VAF variants be interpreted?
    Low VAFs often suggest subclonal populations that might not be targeted by current therapies, or they can represent early, emergent resistance mutations

    FAQ #2 –

    What are actionable mutations in NGS reports?
    Actionable mutations in NGS reports are specific tumor DNA alterations that, when identified, predict a patient’s response to a targeted therapy

    FAQ #3 –

    How are VUS reported and interpreted?
    A Variant of Uncertain Significance (VUS) is reported when genetic testing identifies a DNA change, but insufficient or conflicting evidence exists to classify it as clearly disease-causing or benign. These variants, often found in 30% of hereditary cancer panels, are generally considered inconclusive, and should not be used to guide clinical management or treatment

    FAQ #4 –

    How should an NGS report be read?
    Your Dr is trained to read & interpret an NGS report. An NGS (Next-Generation Sequencing) report should be read by first reviewing the patient and sample details, then focusing on the executive summary to identify actionable genetic variants

    FAQ #5 –

    What is NGS?
    NGS enables scientists and researchers to perform high-throughput sequencing of patient DNA samples, often to identify genetic variation associated with diseases

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