FAQ #1 –
What is the minimum tissue requirement for NGS?
The minimum tissue requirement for NGS generally requires 10–50 ng of DNA, though some targeted assays can function with as little as 1–10 ng, depending on the panel used by the testing lab.
FAQ #2 –
Why do tissue NGS tests fail?
NGS tests in oncology fail in approximately 20–40% of cases, with most these failures caused due to pre-analytical issues related to tissue quantity or quality
FAQ #3 –
How does FFPE age affect sequencing quality?
Older FFPE samples generally produce lower-quality sequencing data due to increased nucleic acid degradation, lower yields, and shorter fragment sizes, particularly in samples over 5–10 years old